Table 2.
Estimated Parameters for GFP-Ras2, GFP-Ras2C318S and GFP-PMA1 expressed as mean (% CV), without including the squares of residuals bleach frame and the immediate postbleach frame intensities in the cost function.
| Strain | ROI size | Model1 | Model2 | |||
|---|---|---|---|---|---|---|
| D (μm2/s) | α(s−1) | D (μm2/s) | α(s−1) | koff (s−1) | ||
| GFP-Ras2 | 1 μm × 1 μm | 0.0705* (4.92) | 29.60* (12.26) | 0.0625 (16.47) | 26.95 (14.65) | 0.0240 (137.95) |
| 0.5 μm × 0.5 μm | 0.0730* (8.39) | 27.88* (14.52) | 0.0976# | 50.07# | 0# | |
| GFP- Ras2C318S | 1 μm × 1 μm | 0.3154* (25.73) | 132.53* (190.08) | 0.3154 (54.96) | 130.83 (262) | 2.5e-4 (6.55e4) |
| 0.5 μm × 0.5 μm | 0.2221* (43.03) | 28.51* (92.12) | 0.2220 (64.35) | 28.17 (870.4) | 2.43e-7 (4.75e9) | |
| GFP-PMA1 | 1 μm × 1 μm | 4.34e-4 | 17.55 | - | - | - |
*
Cannot reject the null hypothesis that these parameters are not distinguishable from each other between two different ROI sizes for the same strain at 95% significance level
#
optimizer hit the lower bound for koff