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. Author manuscript; available in PMC: 2010 Jul 6.
Published in final edited form as: Eur J Immunol. 2008 Dec;38(12):3388–3394. doi: 10.1002/eji.200838282

FIG. 3. Siglec-H− pDCs exhibit high plasticity to differentiate into CD11b+ cDCs.

FIG. 3

BM pDCs were isolated from uninfected or LCMV Cl 13-infected mice and further divided into two subpopulations based on their expression of Ly49Q (A) or Siglec-H (B). Upper panels, pre-sort gating for Ly49Q and Siglec-H staining. Middle panels, purity of FACS-purified populations from LCMV-infected mice. Lower panels, frequency of pDC-derived CD11b+ cDCs 4 days after Flt3L-culture. (C) Siglec-H− pDCs from LCMV Cl 13 infected mice were isolated and cultured for 4 days in the presence of Flt3L. Numbers within regions indicate the frequency of CD11b+ cDCs and Siglec-H+ DCs derived in the culture. (D) Siglec-H expression on BM pDCs from WT and IFN-αβR−/− mice at day 3 p.i. with LCMV Cl 13. Each representative data set was obtained from at least 3 independent experiments.