Table 2.
Odds Ratios (ORs) and 95% Confidence Intervals (CIs) for Biliary Stones and Cancer in Relation to Polymorphisms of Lipid Metabolism Genes
| Biliary Tract Cancers | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype | Controls | Biliary Stones 1 | Gallbladder2 | Bile duct3 | Ampulla of Vater3 | |||||||||||||
| n | (%) | n | (%) | OR4 | (95% CI)4 | n | (%) | OR4 | (95% CI)4 | n | (%) | OR4 | (95% CI)4 | n | (%) | OR4 | (95% CI)4 | |
| TOTAL | 779 | (100.0) | 880 | (100.0) | - | - | 235 | (100.0) | - | - | 125 | (100.0) | - | - | 46 | (100.0) | - | - |
| APOE | ||||||||||||||||||
| IVS1+69C>G (rs440446) | ||||||||||||||||||
| CC | 319 | (41.4) | 326 | (37.4) | 1.00 | - | 85 | (36.8) | 1.00 | - | 32 | (25.8) | 1.00 | - | 10 | (21.7) | 1.00 | - |
| GC | 355 | (46.1) | 425 | (48.7) | 1.25 | (0.99–1.57) | 113 | (48.9) | 1.21 | (0.87–1.67) | 72 | (58.1) | 2.01 | (1.29–3.14) | 24 | (52.2) | 2.09 | (0.98–4.44) |
| GG | 96 | (12.5) | 121 | (13.9) | 1.43 | (1.01–2.02) | 33 | (14.3) | 1.32 | (0.83–2.10) | 20 | (16.1) | 2.07 | (1.13–3.79) | 12 | (26.1) | 3.96 | (1.66–9.46) |
| GC+GG | 451 | (58.6) | 546 | (62.6) | 1.29 | (1.03–1.60) | 146 | (63.2) | 1.23 | (0.91–1.67) | 92 | (74.2) | 2.03 | (1.32–3.11) | 36 | (78.3) | 2.48 | (1.21–5.08) |
| P 5 | 0.02 | 0.18 | 0.003 | 0.002 | ||||||||||||||
| APOB | ||||||||||||||||||
| EX26-3573T>C (rs676210) | ||||||||||||||||||
| TT | 439 | (56.7) | 474 | (53.9) | 1.00 | - | 137 | (58.5) | 1.00 | - | 63 | (50.4) | 1.00 | - | 26 | (57.8) | 1.00 | - |
| TC | 288 | (37.2) | 348 | (39.6) | 1.12 | (0.89–1.40) | 80 | (34.2) | 0.91 | (0.66–1.25) | 57 | (45.6) | 1.38 | (0.94–2.04) | 14 | (31.1) | 0.84 | (0.43–1.64) |
| CC | 47 | (6.1) | 57 | (6.5) | 1.02 | (0.66–1.58) | 17 | (7.3) | 1.15 | (0.64–2.08) | 5 | (4.0) | 0.74 | (0.29–1.94) | 5 | (11.1) | 1.89 | (0.69–5.18) |
| TC+CC | 335 | (43.3) | 405 | (46.1) | 1.10 | (0.89–1.37) | 97 | (41.5) | 0.94 | (0.69–1.27) | 62 | (49.6) | 1.30 | (0.89–1.89) | 19 | (42.2) | 0.99 | (0.54–1.81) |
| P 5 | 0.50 | 0.94 | 0.47 | 0.67 | ||||||||||||||
| IVS23-79T>C (rs673548) | ||||||||||||||||||
| TT | 407 | (56.4) | 446 | (54.5) | 1.00 | - | 129 | (58.6) | 1.00 | - | 59 | (50.9) | 1.00 | - | 26 | (60.5) | 1.00 | - |
| TC | 266 | (36.9) | 320 | (39.1) | 1.09 | (0.86–1.37) | 76 | (34.5) | 0.92 | (0.66–1.27) | 52 | (44.8) | 1.35 | (0.90–2.02) | 13 | (30.2) | 0.78 | (0.40–1.56) |
| CC | 48 | (6.7) | 53 | (6.5) | 0.92 | (0.59–1.42) | 15 | (6.8) | 0.97 | (0.52–1.80) | 5 | (4.3) | 0.72 | (0.27–1.87) | 4 | (9.3) | - | - |
| TC+CC | 314 | (40.6) | 373 | (45.5) | 1.06 | (0.85–1.32) | 91 | (41.4) | 0.93 | (0.68–1.26) | 57 | (49.1) | 1.25 | (0.85–1.86) | 17 | (39.5) | 0.87 | (0.46–1.63) |
| P 5 | 0.86 | 0.69 | 0.60 | 0.62 | ||||||||||||||
| IVS6+360C>T (rs520354) | ||||||||||||||||||
| CC | 515 | (67.2) | 602 | (69.6) | 1.00 | - | 157 | (67.1) | 1.00 | - | 71 | (57.7) | 1.00 | - | 32 | (69.6) | 1.00 | - |
| TC | 234 | (30.6) | 242 | (28.0) | 0.82 | (0.65–1.04) | 69 | (29.5) | 0.93 | (0.67–1.29) | 46 | (37.4) | 1.41 | (0.94–2.10) | 14 | (30.4) | 0.96 | (0.50–1.84) |
| TT | 17 | (2.2) | 21 | (2.4) | 0.97 | (0.48–1.95) | 8 | (3.4) | 1.51 | (0.63–3.60) | 6 | (4.9) | 2.51 | (0.96–6.57) | 0 | 0 | - | - |
| TC+TT | 251 | (32.8) | 263 | (30.4) | 0.83 | (0.66–1.05) | 77 | (32.9) | 0.97 | (0.71–1.33) | 52 | (42.3) | 1.48 | (1.01–2.18) | 14 | (30.4) | 0.90 | (0.47–1.71) |
| P 5 | 0.15 | 0.95 | 0.02 | 0.74 | ||||||||||||||
| EX4+56C>T (rs1367117) | ||||||||||||||||||
| CC | 564 | (73.6) | 650 | (75.3) | 1.00 | - | 182 | (78.1) | 1.00 | - | 81 | (65.9) | 1.00 | - | 32 | (71.1) | 1.00 | - |
| TC | 192 | (25.1) | 195 | (22.6) | 0.84 | (0.66–1.08) | 43 | (18.5) | 0.68 | (0.47–0.99) | 40 | (32.5) | 1.44 | (0.95–2.17) | 12 | (26.7) | 1.13 | (0.57–2.24) |
| TT | 10 | (1.3) | 18 | (2.1) | 1.39 | (0.61–3.17) | 8 | (3.4) | 2.54 | (0.97–6.70) | 2 | (1.6) | - | - | 1 | (2.2) | - | - |
| TC+TT | 202 | (26.4) | 213 | (24.7) | 0.87 | (0.69–1.11) | 51 | (21.9) | 0.77 | (0.54–1.09) | 42 | (34.1) | 1.44 | (0.96–2.15) | 13 | (28.9) | 0.85 | (0.65–1.10) |
| P 5 | 0.44 | 0.44 | 0.08 | 0.67 | ||||||||||||||
| LDLR | ||||||||||||||||||
| IVS9-30C>T (rs1003723) | ||||||||||||||||||
| CC | 565 | (72.6) | 636 | (72.6) | 1.00 | - | 170 | (72.3) | 1.00 | - | 80 | (64.5) | 1.00 | - | 39 | (84.8) | 1.00 | - |
| TC | 199 | (25.6) | 223 | (25.5) | 0.98 | (0.77–1.25) | 61 | (26.0) | 1.02 | (0.73–1.44) | 39 | (31.5) | 1.38 | (0.91–2.10) | 7 | (15.2) | 0.50 | (0.22–1.14) |
| TT | 14 | (1.8) | 17 | (1.9) | 1.02 | (0.47–2.22) | 4 | (1.7) | - | - | 5 | (4.0) | 2.53 | (0.89–7.22) | 0 | 0 | - | - |
| TC+TT | 213 | (27.4) | 240 | (27.4) | 0.98 | (0.78–1.25) | 65 | (27.7) | 1.02 | (0.73–1.41) | 44 | (35.5) | 1.46 | (0.98–2.18) | 7 | (15.2) | 0.47 | (0.21–1.06) |
| P 5 | 0.92 | 0.93 | 0.04 | 0.07 | ||||||||||||||
| EX10+55G>A (rs5927) | ||||||||||||||||||
| GG | 307 | (39.9) | 356 | (41.0) | 1.00 | - | 79 | (33.9) | 1.00 | - | 54 | (43.2) | 1.00 | - | 17 | (37.0) | 1.00 | - |
| GA | 348 | (45.2) | 382 | (44.0) | 0.94 | (0.74–1.18) | 120 | (51.5) | 1.34 | (0.97–1.85) | 52 | (41.6) | 0.85 | (0.56–1.28) | 17 | (37.0) | 0.88 | (0.44–1.76) |
| AA | 115 | (14.9) | 130 | (15.0) | 0.94 | (0.68–1.30) | 34 | (14.6) | 1.10 | (0.70–1.74) | 19 | (15.2) | 0.94 | (0.53–1.65) | 12 | (26.0) | 1.88 | (0.87–4.07) |
| GA+AA | 463 | (60.1) | 512 | (59.0) | 0.94 | (0.75–1.17) | 154 | (66.1) | 1.28 | (0.95–1.75) | 71 | (56.8) | 0.90 | (0.61–1.32) | 29 | (63.0) | 1.16 | (0.63–2.15) |
| P 5 | 0.62 | 0.35 | 0.66 | 0.18 | ||||||||||||||
| EX15-80G>A (rs14158) | ||||||||||||||||||
| GG | 696 | (89.6) | 794 | (90.7) | 1.00 | - | 204 | (86.8) | 1.00 | - | 114 | (91.9) | 1.00 | - | 42 | (91.3) | 1.00 | - |
| GA | 76 | (9.8) | 79 | (9.0) | 0.85 | (0.59–1.22) | 30 | (12.8) | 1.29 | (0.82–2.02) | 10 | (8.1) | 0.80 | (0.40–1.60) | 4 | (8.7) | 0.87 | (0.30–2.49) |
| AA | 5 | (0.6) | 2 | (0.2) | 0.30 | (0.06–1.55) | 1 | (0.4) | - | - | 0 | 0 | - | - | 0 | 0 | - | - |
| GA+AA | 81 | (10.4) | 81 | (9.3) | 0.81 | (0.57–1.15) | 31 | (13.2) | 1.24 | (0.80–1.94) | 10 | (8.1) | 0.75 | (0.38–1.50) | 4 | (8.7) | 0.81 | (0.28–2.31) |
| P 5 | 0.16 | 0.33 | 0.42 | 0.70 | ||||||||||||||
| IVS17-42A>G (rs6413504) | ||||||||||||||||||
| AA | 411 | (53.4) | 448 | (51.2) | 1.00 | - | 120 | (51.3) | 1.00 | - | 59 | (47.2) | 1.00 | - | 25 | (54.3) | 1.00 | - |
| GA | 291 | (37.8) | 359 | (41.0) | 1.19 | (0.95–1.49) | 98 | (41.9) | 1.19 | (0.87–1.62) | 56 | (44.8) | 1.34 | (0.90–1.99) | 15 | (32.6) | 0.84 | (0.43–1.62) |
| GG | 67 | (8.8) | 68 | (7.8) | 0.94 | (0.63–1.39) | 16 | (6.8) | 0.81 | (0.45–1.46) | 10 | (8.0) | 1.04 | (0.51–2.13) | 6 | (13.0) | 1.42 | (0.56–3.60) |
| GA+GG | 358 | (46.6) | 427 | (48.8) | 1.14 | (0.92–1.41) | 114 | (48.7) | 1.12 | (0.83–1.50) | 66 | (52.8) | 1.28 | (0.88–1.87) | 21 | (45.7) | 0.95 | (0.52–1.72) |
| P 5 | 0.52 | 0.87 | 0.38 | 0.78 | ||||||||||||||
| EX18+88G>A (rs5930) | ||||||||||||||||||
| GG | 290 | (37.5) | 320 | (36.7) | 1.00 | - | 86 | (36.9) | 1.00 | - | 49 | (39.5) | 1.00 | - | 12 | (26.1) | 1.00 | - |
| GA | 364 | (47.2) | 416 | (47.7) | 1.05 | (0.83–1.33) | 108 | (46.4) | 1.01 | (0.73–1.40) | 56 | (45.2) | 0.91 | (0.60–1.38) | 21 | (45.7) | 1.38 | (0.67–2.86) |
| AA | 118 | (15.3) | 136 | (15.6) | 1.04 | (0.75–1.44) | 39 | (16.7) | 1.12 | (0.72–1.73) | 19 | (15.3) | 0.95 | (0.54–1.69) | 13 | (28.3) | 2.71 | (1.19–6.12) |
| GA+AA | 482 | (62.4) | 552 | (63.3) | 1.05 | (0.84–1.31) | 147 | (63.1) | 1.04 | (0.76–1.41) | 75 | (60.5) | 0.92 | (0.62–1.36) | 34 | (73.9) | 1.70 | (0.87–3.34) |
| P 5 | 0.74 | 0.66 | 0.78 | 0.02 | ||||||||||||||
| LPL | ||||||||||||||||||
| IVS5-540C>T (rs263) | ||||||||||||||||||
| CC | 485 | (62.6) | 539 | (61.3) | 1.00 | - | 151 | (64.3) | 1.00 | - | 79 | (63.7) | 1.00 | - | 28 | (60.9) | 1.00 | - |
| TC | 254 | (32.8) | 308 | (35.0) | 1.17 | (0.93–1.47) | 76 | (32.3) | 0.97 | (0.71–1.34) | 39 | (31.5) | 0.94 | (0.62–1.42) | 18 | (39.1) | 1.20 | (0.65–2.21) |
| TT | 36 | (4.6) | 32 | (3.6) | 0.68 | (0.41–1.13) | 8 | (3.4) | 0.69 | (0.31–1.52) | 6 | (4.8) | 1.02 | (0.42–2.51) | 0 | 0 | - | - |
| TC+TT | 290 | (37.4) | 340 | (38.7) | 1.10 | (0.88–1.36) | 84 | (35.7) | 0.94 | (0.69–1.27) | 45 | (36.3) | 0.95 | (0.64–1.41) | 18 | (39.1) | 1.05 | (0.57–1.94) |
| P 5 | 0.95 | 0.50 | 0.86 | 0.85 | ||||||||||||||
| ALOX5 | ||||||||||||||||||
| IVS3+100G>A (rs2029253) | ||||||||||||||||||
| GG | 286 | (37.0) | 319 | (36.4) | 1.00 | - | 84 | (35.9) | 1.00 | - | 48 | (38.7) | 1.00 | - | 15 | (32.6) | 1.00 | - |
| GA | 376 | (48.5) | 432 | (49.3) | 1.09 | (0.86–1.37) | 113 | (48.3) | 1.05 | (0.76–1.44) | 60 | (48.4) | 0.95 | (0.63–1.43) | 25 | (54.3) | 1.24 | (0.64–2.39) |
| AA | 112 | (14.5) | 125 | (14.3) | 1.12 | (0.80–1.57) | 37 | (15.8) | 1.16 | (0.74–1.82) | 16 | (12.9) | 0.85 | (0.46–1.56) | 6 | (13.0) | 0.98 | (0.37–2.59) |
| GA+AA | 488 | (63.1) | 557 | (63.6) | 1.09 | (0.88–1.36) | 150 | (64.1) | 1.07 | (0.79–1.46) | 76 | (61.3) | 0.93 | (0.63–1.37) | 31 | (67.4) | 1.17 | (0.62–2.22) |
| P 5 | 0.43 | 0.53 | 0.60 | 0.85 | ||||||||||||||
1
Biliary stone cases include gallstone and bile duct stones. Biliary stone cases compared with controls without biliary stones (n=586)
2
Gallbadder cancer cases compared with population controls who had a gallbladder (n=730)
3
Bile duct and ampulla of Vater cancer cases compared to all population controls (n=779)
4
Adjusted for age
5
Test of trend for the number of copies of the variant allele (0,1,2);
Pearson chi-square for absence or presence of the variant allele if variant genotype has n < 5
Note: Total number of subjects per SNP may vary because of missing values