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. Author manuscript; available in PMC: 2010 Nov 11.
Published in final edited form as: Curr Biol. 2010 Apr 22;20(9):856–860. doi: 10.1016/j.cub.2010.03.028

Figure 3. DNA synthesis requires active centrosomal cyclin E-Cdk2.

Figure 3

(A) In cells expressing the wild-type GFP-CLS, localization of Myc-tagged cyclin E SWNQ-A-S180D without (left) and with (right) fusion to the PACT domain was analyzed by immunofluorescence as in Fig. 2A. Arrowheads mark centrosomes. Inset: enlarged image of merged centrosomal area. Scale bars: 5μM.

(B) DNA synthesis requires Cdk activity at the centrosome. The cyclin E double mutant SWNQ-A-S180D deficient in both centrosomal localization and Cdk binding was assessed for restoration of BrdU incorporation in CLS-expressing cells with or without fusion to the PACT domain, as indicated. Error bars represent mean ± SD (n=5).