Table 1. Plasmids used in this study.
Plasmid | Description | Marker |
pDEP005 | SR, pRS305-Phac1-h5′-GFP-h3′ | LEU2 |
pDEP007 | Ire1-GFP, wt IRE1-GFP in pRS305 | LEU2 |
pDEP010 | Ire1-mCherry, wt IRE1 in pRS306 | URA3 |
pDEP017 | TR, pRS304-4×UPRE-GFP | TRP1 |
pDEP044 | 2 µ plasmid, wt IRE1 in pRS423 | HIS3 |
pDEP045 | 2 µ plasmid, Ire1bipless in pRS423 | HIS3 |
pDEP049 | Ire1bipless, pRS306-PIre1-Ire1bipless | URA3 |
pDEP053 | Ire1bipless-GFP, pRS306-PIre1-Ire1bipless-GFP | URA3 |
pDEP060 | Ire1biipless-mCherrry, pRS305-Ire1bipless-mCherry | LEU2 |