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. 2010 Jun 14;9:45. doi: 10.1186/1475-2859-9-45

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Copyright ©2010 Groisillier et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Assessing soluble and insoluble protein expression levels in the medium throughput strategy. a. SDS-PAGE of Insoluble (I) and soluble (S) crude cellular extracts of the E. coli expression cultures. Targets 50 to 60 are shown. Black circles surround bands of correct size for a given target. b. Western-blot using anti-His antibody of selected targets highlighted after purification of soluble cell lysis extracts on mini-affinity (His) columns. Selected targets between 156 and 181 are shown. Pink circles highlight bands of correct size for a given target.