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. 2010 Jun 14;9:45. doi: 10.1186/1475-2859-9-45

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Copyright ©2010 Groisillier et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Up-scale and purification of targets from P. abyssi. a. SDS-PAGE gel of up-scaled and purified HIS-tagged targets F75, F124, F127 and F167 before and after final polishing on Co²⁺specific beads (SN = Supernatant of cell culture; B = after incubation with Dynabeads TALON; M = protein markers; F75 = test line; NI = F75 without induction). b. 12% SDS-PAGE analysis of a P. abyssi RadA (PAB0164, target 92 round R1) pull-down experiment. Proteins identified using an Ultraflex MALDI-TOF/TOF instrument (Bruker Daltonics) are indicated with arrows.