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Up-scale purification and crystallization of R-Z3597 from Zobellia galactanivorans. a. SDS-PAGE gel of up-scaled and purified His-tagged target Zg3597. Lane 1, soluble extract; lane 2, purified R-Z3597 by affinity chromatography using a Ni2+-charged resin; lane 3, R-Z3597 purified by size exclusion gel chromatography; M, standard molecular weight markers. b. Orthorhombic crystals of Zg3597 (0.1 × 0.1 × 0.5 μm3)
