Table 2.
Comparison of obtained results covering three domains of living organisms
| Eukarya | Bacteria | Archaea | Total | ||||||
|---|---|---|---|---|---|---|---|---|---|
| pFO4 | pGEX | pFO4 | pGEX | pFO4 | pGEX | pFO4 | pGEX | combinedc | |
| number of soluble proteins | 14 | 13 | 13 | 25 | 18 | 26 | 45 | 64 | 84 |
| number of insoluble proteins | 10 | 19 | 4 | 5 | 12 | 24 | 26 | 48 | 34 |
| no expressiona | 42 | 37 | 11 | 2 | 31 | 11 | 84 | 50 | 52 |
| no cloneb | 8 | 5 | 4 | 0 | 9 | 12 | 37 | 30 | 22 |
| percentage of soluble proteins | 7% | 7% | 7% | 13% | 9% | 14% | 23% | 33% | 44% |
| percentage of soluble + insoluble proteins | 13,00% | 17% | 9% | 16% | 16% | 26% | 37% | 58% | 61% |
a number of targets for which no expected bands were visualized on SDS-PAGE gels
b number of target genes did not clone in expression vectors
c number of different soluble proteins obtained in pGEX or in pFO4 vectors