Table 3.
Escherichia coli strains and plasmids used in this study.
| Genotypes and relevant properties | Sources or references | |
|---|---|---|
| Strains | ||
| DH5α | F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG ϕ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ- | Promega, USA |
| BL21 | F- ompT gal dcm lon hsdSB(rB- mB-) | Novagen, USA |
| BL21 (DE3) | F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Novagen, USA |
| Rosetta | F- ompT hsdSB(RB- mB-) gal dcm | Novagen, USA |
| Rosetta (DE3) | F- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Novagen, USA |
| Origami | F- ompT hsdSB rB- mB-) gal dcm lacY1 ahpC gor522::Tn10 (TcR) trxB::kan pAR5615 (ApR) | Novagen, USA |
| Origami (DE3) | F- ompT hsdSB rB- mB-) gal dcm lacY1 ahpC gor522::Tn10 (TcR) trxB::kan pAR5615 (ApR) λ(DE3[lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) | Novagen, USA |
| Plasmids | ||
| pGEX-4T1 | AmpR, tac promoter, GST.Tag | GE HealthCare, USA |
| pFO4 | AmpR, T7lac promoter, His.Tag | This Study |