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. 2010 May 26;5:5. doi: 10.1186/1750-2187-5-5

Figure 1.

Figure 1

Co-localization of intracellular Zn2+ and ER fluorescence. Images of cultured cortical neurons co-labeled with the intracellular fluorescent Zn2+ indicators and a live cell marker for the ER. A. Fluorescent Zn2+ indicator Newport Green AM co-localizes with the ER marker ER-Tracker Red. B. Fluorescent Zn2+ indicator ZinPyr-1 co-localizes with the ER marker ER-Tracker Red. Cells were incubated with 10 μM of the specified fluorescent Zn2+ indicator and 1 μM ER-Tracker Red then imaged using a LSM510 confocal microscope equipped with a 100x/1.3NA objective.