Figure 3.

SDS-PAGE of the purified pET32a/DPV-gE and Western blotting analysis. A. The SDS-PAGE analysis of the purity of pET32a/DPV-gE. Lane 1-2, the recombinant protein was purified by the Ni²⁺-NTA agarose gel; Lane 3, the pET32a/DPV-gE before induction in E. coli Rosseta; Lane 4, the pET32a/DPV-gE after induction in E. coli Rosseta; 5: Protein marker. B. The immunogenicity of the recombinant gE protein was analyzed by western blotting with the rabbit anti-DPV IgG.. M: Prestained Protein Molecular Weight Marker; Lane 1, Expression of the recombinant plasmid pET32a/DPV-gE uninduced; Lane 2, Expression of the recombinant plasmid pET32a/DPV-gE induced by IPTG; C. The recombinant protein gE was recognized with the pET32a/DPV-gE antiserum. Lane 3, Expression of the recombinant plasmid pET32a/DPV-gE induced by IPTG; Lane 4, Expression of the recombinant plasmid pET32a/DPV-gE uninduced; Lane 5, the recombinant gE protein was analyzed with the pre-immune serum.