Figure 5.

Intracellular location and distribution of DPV gE analyzed by indirect immunofluorescence. F1, Mock-infected cells were fixed; F2, DPV-infected were fixed at 5.5 h post-infection; F3-7, DPV-infected cells were fixed at 5.5 h, 9 h, 36 h, 48 h, and 60 h post-infection, the sample F2 was incubated with pre-immune serum, the other samples were incubated with the pET32a/DPV-gE antiserum. The merged fluorescence microscopy images of DEF are shown in panels F1 to F7 with high magnification (600×).