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. 2010 Jun 8;10:166. doi: 10.1186/1471-2180-10-166

Figure 4.

Figure 4

Quantitation of proteins by their tryptic peptide fragments. A tryptic peptide fragment FTGWYDVDLSEK (MW 1459.81) from S. Enteritidis phosphoglyceromutase was analyzed for the level of downregulation. A peak at m/z 1473 represents 15N-labeled population (upper spectrum), which does not appear in the unlabeled population. The ratio of two peak intensities (27 and 17, respectively) represents a relative protein expression level of 0.6, or a 40% downregulation.