Table 1.
MALDI-ToF analysis and identification of SE2472 proteins.
| Locus Tag |
Description |
Gene |
Mass (KDa) |
pI |
Coverage |
|---|---|---|---|---|---|
| PSLT011 | Dlp (SrgA) | srgA | 24.74 | 8.58 | 38% |
| STM0007 | Transaldolase B | talB | 35.15 | 5.09 | 19% |
| STM0012 | Chaperone protein dnaK (Heat shock protein 70) | dnaK | 69.2 | 4.84 | 22% |
| STM0013 | Chaperone protein dnaJ | dnaJ | 41.31 | 8.41 | 25% |
| STM0093 | Organic solvent tolerance protein | Imp | 89.8 | 5.21 | 23% |
| STM0102 | L-arabinose isomerase | araA | 55.89 | 5.88 | 23% |
| STM0158 | Aconitate hydratase 2 | acnB | 82.2 | 5.35 | 29% |
| STM0217 | Elongation factor Ts | tsf | 33.18 | 5.16 | 41% |
| STM0316 | Aminoacyl-histidine dipeptidase | pepD | 52.69 | 5.17 | 15% |
| STM0432 | Phosphonoacetaldehyde hydrolase | phnX | 28.57 | 5.58 | 41% |
| STM0435 | Nucleotide-binding protein | yajQ | 18.31 | 5.6 | 52% |
| STM0447 | Trigger factor | tig | 48.02 | 4.84 | 23% |
| STM0488 | Adenylate kinase | adk | 23.49 | 5.53 | 51% |
| STM0536 | Peptidyl-prolyl cis-trans isomerase B | ppiB | 18.13 | 5.52 | 45% |
| STM0608 | Chain T, crystal structure of Ahpc | ahpC | 20.64 | 5.03 | 24% |
| STM0730 | Citrate synthase | gltA | 48.11 | 6.35 | 24% |
| STM0772 | Phosphoglyceromutase | gpmA | 28.48 | 5.78 | 19% |
| STM0776 | UDP-galactose 4-epimerase | galE | 37.28 | 5.79 | 31% |
| STM0781 | Molybdate transporter periplasmic protein | modA | 27.5 | 6.53 | 67% |
| STM0794 | Biotin synthase | bioB | 38.8 | 5.42 | 53% |
| STM0830 | Glutamine-binding periplasmic protein precursor | glnH | 27.23 | 8.74 | 67% |
| STM0877 | Putrescine-binding periplasmic protein precursor | potF | 41 | 6.02 | 35% |
| STM0999 | Outer membrane protein F precursor | ompF | 40.05 | 4.73 | 28% |
| STM1091 | Secretory Effector Protein | SopB | 61.93 | 9.27 | 42% |
| STM1220 | N-acetyl-D-glucosamine kinase | nagK | 33.06 | 5.09 | 29% |
| STM1231 | DNA-binding response regulator in PhoQ system | phoP | 25.61 | 5.28 | 33% |
| STM1290 | Glyceraldehyde-3-phosphate dehydrogenase | gapA | 36.1 | 6.33 | 29% |
| STM1296 | Putative oxidoreductase | ydjA | 20.13 | 6.75 | 29% |
| STM1302 | Exonuclease III | xthA | 30.79 | 6.19 | 23% |
| STM1303 | Succinylornithine transaminase | astC | 43.72 | 6.13 | 34% |
| STM1310 | NAD synthetase | nadE | 30.57 | 5.36 | 27% |
| STM1378 | Pyruvate kinase I | pykF | 50.66 | 5.66 | 31% |
| STM1431 | Superoxide dismutase | sodB | 21.35 | 5.58 | 35% |
| STM1544 | PhoPQ-regulated protein | pqaA | 59.27 | 6.87 | 20% |
| STM1567 | Alcohol dehydrogenase | adhP | 35.49 | 5.8 | 42% |
| STM1589 | Putative NADP-dependent oxidoreductase | yncB | 39.2 | 5.6 | 23% |
| STM1641 | ATP-dependent helicase | hrpA | 148.71 | 8.22 | 15% |
| STM1661 | Putative universal stress protein | ydaA | 35.62 | 5.17 | 66% |
| STM1682 | Thiol peroxidase | tpx | 18.19 | 4.93 | 54% |
| STM1714 | DNA topoisomerase I | topA | 97.03 | 8.56 | 26% |
| STM1727 | Tryptophan synthase | trpA | 28.65 | 5.28 | 20% |
| STM1746.S | Chain A, structural basis of multispecificity in Oppa | oppA | 58.77 | 5.85 | 29% |
| STM1796 | Trehalase, periplasmic | treA | 63.6 | 5.19 | 63% |
| STM1886 | Glucose-6-phosphate 1-dehydrogenase | zwf | 55.92 | 5.52 | 26% |
| STM1923 | Chemotaxis protein motA | motA | 32.08 | 5.47 | 31% |
| STM1954 | Cystine-binding periplasmic protein precursor | fliY | 28.79 | 8.81 | 23% |
| STM1959 | Flagellin | fliC | 51.62 | 4.79 | 56% |
| STM2104 | Phosphomannomutase in colanic acid gene cluster | cpsG | 50.02 | 5.18 | 20% |
| STM2167 | NADH independent D-lactate dehydrogenase | dld | 65.05 | 6.47 | 31% |
| STM2190 | D-galactose binding periplasmic protein | mglB | 35.81 | 5.81 | 31% |
| STM2203 | Endonuclease IV | nfo | 31.2 | 5.17 | 45% |
| STM2205 | Fructose-1-phosphate kinase | fruK | 33.71 | 5.36 | 39% |
| STM2282 | Glycerophosphodiester phosphodiesterase | glpQ | 40.42 | 5.66 | 24% |
| STM2337 | Acetate kinase | ackA | 43.26 | 5.93 | 21% |
| STM2347 | Putative phosphoesterase | yfcE | 19.91 | 5.93 | 43% |
| STM2362 | Amidophosphoribosyltransferase | purF | 56.56 | 5.51 | 23% |
| STM2501 | Polyphosphate kinase | ppk | 80.46 | 8.7 | 30% |
| STM2549 | Anaerobic sulfide reductase | asrB | 30.61 | 6.24 | 28% |
| STM2647 | Uracil-DNA glycosylase | ung | 25.48 | 6.56 | 67% |
| STM2829 | DNA strand exchange and recombinant protein | recA | 37.94 | 5.08 | 28% |
| STM2864 | Iron transporter protein, fur regulated | sitD | 33.7 | 7.84 | 41% |
| STM2882 | Secretory Effector Protein | sipA | 73.94 | 6.41 | 35% |
| STM2884 | Translocation Machinery Component | sipC | 42.98 | 8.88 | 38% |
| STM2924 | RNA polymerase sigma factor rpoS | rpoS | 37.93 | 4.86 | 29% |
| STM2952 | Enolase | eno | 36.24 | 5.13 | 30% |
| STM2976 | L-fucose isomerase | fucI | 64.77 | 5.6 | 31% |
| STM2993 | Exonuclease V, alpha chain | recD | 67.05 | 8.02 | 36% |
| STM3068 | Fructose-bisphosphate aldolase | fba | 39.3 | 5.68 | 25% |
| STM3069 | Phosphoglycerate kinase | pgk | 41.28 | 5.09 | 38% |
| STM3186 | Outer membrane channel protein | tolC | 53.39 | 5.42 | 31% |
| STM3219 | 2,4-dieonyl-CoA reductase | fadH | 73.13 | 6.55 | 35% |
| STM3225 | Serine/threonine transporter | sstT | 43.41 | 8.43 | 33% |
| STM3294 | Phosphoglucosamine mutase | glmM | 47.44 | 5.74 | 32% |
| STM3342 | Stringent starvation protein A | sspA | 32.05 | 5.22 | 19% |
| STM3359 | Malate dehydrogenase | mdh | 32.63 | 6.01 | 22% |
| STM3380 | Acetyl CoA carboxylase | accC | 49.26 | 6.52 | 28% |
| STM3401 | Shikimate dehydrogenase | aroE | 29.29 | 5.73 | 51% |
| STM3445 | Elongation factor Tu | tuf | 43.26 | 5.3 | 32% |
| STM3446 | Elongation factor G | fusA | 77.72 | 5.17 | 23% |
| STM3484 | DNA adenine methylase | dam | 32.03 | 8.93 | 26% |
| STM3496 | Putative hydrolase | yrfG | 72.4 | 5.23 | 19% |
| STM3500 | Phosphoenolpyruvate carboxykinase | pckA | 59.9 | 5.67 | 28% |
| STM3502 | Osmolarity response regulator | ompR | 27.35 | 6.04 | 31% |
| STM3557 | Glycerol-3-phosphatase transporter binding protein | ugpB | 48.49 | 6.97 | 15% |
| STM3612 | 2-dehydro-3-deoxygluconokinase | kdgK | 34.35 | 5.01 | 17% |
| STM3884 | D-ribose periplasmic binding protein | rbsB | 30.9 | 8.54 | 38% |
| STM3968 | Uridine phosphorylase | udp | 27.38 | 6.32 | 34% |
| STM3997 | Thiol:disulfide interchange protein | dsbA | 22.9 | 6.3 | 54% |
| STM4029 | Putative acetyltransferase | yiiD | 36.92 | 6.08 | 34% |
| STM4166 | NADH pyrophosphatase | nudC | 29.62 | 5.89 | 48% |
| STM4256 | Single-strand DNA-binding protein | ssb | 19.06 | 5.46 | 34% |
| STM4329 | Co-chaperonin groES | groES | 10.19 | 5.36 | 56% |
| STM4330 | Chaperonin groEL | groEL | 57.16 | 4.85 | 38% |
| STM4343 | Fumarate reductase | frdA | 65.49 | 5.95 | 19% |
| STM4359 | DNA mismatch repair protein mutL | mutL | 67.76 | 6.51 | 21% |
| STM4414 | Inorganic pyrophosphatase | ppa | 19.68 | 5.01 | 43% |
| STM4513 | Putative permease | yjiG | 16.12 | 7.76 | 61% |
| STM4567 | Deoxyribose-phosphate aldolase | deoC | 27.68 | 5.87 | 47% |
| STM4568 | Thymidine phosphorylase | deoA | 47 | 4.96 | 38% |
| STM4569 | Phosphopentomutase | deoB | 44.24 | 5.15 | 52% |
| STM4598 | Two-component response regulator | arcA | 45.56 | 5.47 | 58% |
| STY2300 | CDP-6-deoxy-D-xylo-4-hexulose-3-dehydrase | rfbH | 48.1 | 5.27 | 46% |
| STY2300 | CDP-4-keto-6-deoxy-D-glucose-3-dehydrase | ddhC | 48.2 | 5.35 | 39% |