Table 2.
Quantitative analysis of the expression of SE2472 proteins upon exposure to H2O2.
| Locus Tag | Description | Gene | % Change |
|---|---|---|---|
| PSLT011 | Dlp (SrgA) | srgA | 12 ± 2% |
| STM0007 | Transaldolase B | talB | 0% |
| STM0012 | Chaperone protein dnaK (Heat shock protein 70) | dnaK | 56 ± 7% |
| STM0013 | Chaperone protein dnaJ | dnaJ | 38 ± 3% |
| STM0093 | Organic solvent tolerance protein | Imp | 210 ± 30% |
| STM0102 | L-arabinose isomerase | araA | 26 ± 2% |
| STM0158 | Bifunctional aconitate hydratase | acnB | 25 ± 5% |
| STM0217 | Elongation factor Ts | tsf | 21 ± 4% |
| STM0316 | Aminoacyl-histidine dipeptidase | pepD | 9 ± 1% |
| STM0432 | Phosphonoacetaldehyde hydrolase | phnX | 31 ± 3% |
| STM0435 | Nucleotide-binding protein | yajQ | 0% |
| STM0447 | Trigger factor | tig | 11 ± 2% |
| STM0488 | Adenylate kinase | adk | 0% |
| STM0536 | Peptidyl-prolyl cis-trans isomerase B | ppiB | 0% |
| STM0608 | Chain T, crystal structure of Ahpc | ahpC | 0% |
| STM0730 | Citrate synthase | gltA | 42 ± 5% |
| STM0772 | Phosphoglyceromutase | gpmA | -40 ± 10% |
| STM0776 | UDP-galactose 4-epimerase | galE | 23 ± 2% |
| STM0781 | Molybdate transporter periplasmic protein | modA | 11 ± 2% |
| STM0794 | Biotin synthase | bioB | 0% |
| STM0830 | Glutamine-binding periplasmic protein precursor | glnH | 10 ± 3% |
| STM0877 | Putrescine-binding periplasmic protein precursor | potF | 11 ± 2% |
| STM0999 | Outer membrane protein F precursor | ompF | 0% |
| STM1091 | Secretory Effector Protein | SopB | -55% ± 7% |
| STM1220 | N-acetyl-D-glucosamine kinase | nagK | 12 ± 3% |
| STM1231 | DNA-binding response regulator in PhoQ system | phoP | 20 ± 6% |
| STM1290 | Glyceraldehyde-3-phosphate dehydrogenase | gapA | 31 ± 3% |
| STM1296 | Putative oxidoreductase | ydjA | -30 ± 5% |
| STM1302 | Exonuclease III | xthA | 0% |
| STM1303 | Succinylornithine transaminase | astC | 41 ± 7% |
| STM1310 | NAD synthetase | nadE | 9 ± 1% |
| STM1378 | Pyruvate kinase I | pykF | 87 ± 12% |
| STM1431 | Superoxide dismutase | sodB | 110 ± 20% |
| STM1544 | PhoPQ-regulated protein | pqaA | 19 ± 2% |
| STM1567 | Alcohol dehydrogenase | adhP | 9 ± 2% |
| STM1589 | Putative NADP-dependent oxidoreductase | yncB | 12 ± 2% |
| STM1641 | ATP-dependent helicase | hrpA | 20 ± 3% |
| STM1661 | Putative universal stress protein | ydaA | 140 ± 20% |
| STM1682 | Probable peroxidase | tpx | 19 ± 2% |
| STM1714 | DNA topoisomerase I | topA | 17 ± 4% |
| STM1727 | Tryptophan synthase | trpA | 37 ± 9% |
| STM1746.S | Chain A, structural basis of multispecificity in Oppa | oppA | 0% |
| STM1796 | Trehalase, periplasmic | treA | 25 ± 3% |
| STM1886 | Glucose-6-phosphate 1-dehydrogenase | zwf | 0% |
| STM1923 | Chemotaxis protein motA | motA | 14 ± 3% |
| STM1954 | Cystine-binding periplasmic protein precursor | fliY | 9 ± 2% |
| STM1959 | Flagellin | fliC | 0% |
| STM2104 | Phosphomannomutase in colanic acid gene cluster | cpsG | 23 ± 6% |
| STM2167 | NADH independent D-lactate dehydrogenase | dld | 16 ± 5% |
| STM2190 | D-galactose binding periplasmic protein | mglB | 34 ± 3% |
| STM2203 | Endonuclease IV | nfo | 0% |
| STM2205 | Fructose-1-phosphate kinase | fruK | 35 ± 3% |
| STM2282 | Glycerophosphodiester phosphodiesterase | glpQ | 15 ± 3% |
| STM2337 | Acetate kinase | ackA | 23 ± 3% |
| STM2347 | Putative phosphoesterase | yfcE | 0% |
| STM2362 | Amidophosphoribosyltransferase | purF | 10 ± 4% |
| STM2501 | Polyphosphate kinase | ppk | 7 ± 3% |
| STM2549 | Anaerobic sulfide reductase | asrB | 0% |
| STM2647 | Uracil-DNA glycosylase | ung | 27 ± 2% |
| STM2829 | DNA strand exchange and recombinant protein | recA | 24 ± 2% |
| STM2864 | Iron transporter protein, fur regulated | sitD | -45 ± 8% |
| STM2882 | Secretory Effector Protein | sipA | 0% |
| STM2884 | Translocation Machinery Component | sipC | 301 ± 30% |
| STM2924 | RNA polymerase sigma factor rpoS | rpoS | 13 ± 2% |
| STM2952 | Enolase | eno | 23 ± 4% |
| STM2976 | L-fucose isomerase | fucI | 0% |
| STM2993 | Exonuclease V, alpha chain | recD | 0% |
| STM3068 | Fructose-bisphosphate aldolase | fba | 52 ± 7% |
| STM3069 | Phosphoglycerate kinase | pgk | 20 ± 3% |
| STM3186 | Outer membrane channel protein | tolC | 0% |
| STM3219 | 2,4-dieonyl-CoA reductase | fadH | 24 ± 3% |
| STM3225 | Serine/threonine transporter | sstT | 23 ± 3% |
| STM3294 | Phosphoglucosamine mutase | glmM | 18 ± 2% |
| STM3342 | Stringent starvation protein A | sspA | -20 ± 4% |
| STM3359 | Malate dehydrogenase | mdh | 36 ± 6% |
| STM3380 | Acetyl CoA carboxylase | accC | 11 ± 2% |
| STM3401 | Shikimate dehydrogenase | aroE | 12 ± 3% |
| STM3445 | Elongation factor Tu | tuf | 0% |
| STM3446 | Elongation factor G | fusA | 9 ± 2% |
| STM3484 | DNA adenine methylase | dam | 26 ± 3% |
| STM3496 | Putative hydrolase | yrfG | 0% |
| STM3500 | Phosphoenolpyruvate carboxykinase | pckA | 330 ± 40% |
| STM3502 | Osmolarity response regulator | ompR | 12 ± 3% |
| STM3557 | Glycerol-3-phosphatase transporter binding protein | ugpB | 0% |
| STM3612 | 2-dehydro-3-deoxygluconokinase | kdgK | 9 ± 2% |
| STM3884 | D-ribose periplasmic binding protein | rbsB | 31 ± 3% |
| STM3968 | Uridine phosphorylase | udp | 11 ± 5% |
| STM3997 | Thiol:disulfide interchange protein | dsbA | 10 ± 5% |
| STM4029 | Putative acetyltransferase | yiiD | 0% |
| STM4166 | NADH pyrophosphatase | nudC | 10 ± 2% |
| STM4256 | Single-strand DNA-binding protein | ssb | 19 ± 2% |
| STM4329 | Co-chaperonin groES | groES | 51 ± 3% |
| STM4330 | Chaperonin groEL | groEL | 43 ± 2% |
| STM4343 | Fumarate reductase | frdA | 40 ± 2% |
| STM4359 | DNA mismatch repair protein mutL | mutL | 41 ± 3% |
| STM4414 | Inorganic pyrophosphatase | ppa | 0% |
| STM4513 | Putative permease | yjiG | -78 ± 15% |
| STM4567 | Deoxyribose-phosphate aldolase | deoC | 0% |
| STM4568 | Thymidine phosphorylase | deoA | -9 ± 2% |
| STM4569 | Phosphopentomutase | deoB | 0% |
| STM4598 | Two-component response regulator | arcA | 10 ± 4% |
| STY2300 | CDP-6-deoxy-D-xylo-4-hexulose-3-dehydrase | rfbH | 0% |
| STY2300 | CDP-4-keto-6-deoxy-D-glucose-3-dehydrase | ddhC | 0% |
Relative expression level in the presence of H2O2 compared against control (in the absence of H2O2) is shown. An average of 10 peaks was used to calculate the mean intensity ratios and the error percentage of each protein spot. The results were the average from triplicate experiments. The limit of detection was arbitrarily set to 5% and any proteins that showed less than 5% change were classified as 0%.