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. 2010 Jun 7;10:263. doi: 10.1186/1471-2407-10-263

Figure 4.

Figure 4

Characterization of epithelial structures in collagen gels after 2 weeks in culture. Co-cultures of MCF7 cells and RMF were characterized using immunohistochemical analyses for luminal epithelial cell origin (Keratin 18), adherens junctions (E-cadherin), and polarization (Sialomucin). No staining was observed for the basement membrane marker type IV collagen. Arrowhead points to fibroblast. Scale bar: 50 μm.