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. 2010 Jun 7;10:263. doi: 10.1186/1471-2407-10-263

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Copyright ©2010 Krause et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of epithelial structures in mixed Matrigel™-collagen gels after two weeks in culture. Co-cultures of MCF7 cells and RMF were characterized using immunohistochemical analyses for luminal epithelial cell origin (Keratin 18), adherens junctions (E-cadherin), and polarization (Sialomucin). No staining was observed for the basement membrane marker type IV collagen. Arrow points to the luminal localization of Sialomucin staining; arrowhead signals fibroblast. Scale bar: 50 μm.