Figure 5. TcCRT binds to live HUVECs and is internalized.

HUVECs were incubated with: A. FITC or 1 µM: B. TcCRT, C. TcCRT-FITC, D. TcCRT-FITC + 10 µM TcCRT, E. TcCRT-FITC and F. TcCRT-FITC + 25 µg/ml fucoidin, for 1h at 37°C, 5% CO₂. The cells were then washed, fixed and analyzed by fluorescence (A–D) or confocal microscopy (E and F). Results are representative of three independent experiments. Original magnification, ×40 (A–D) and ×100 (E and F).