Figure 2. Time course analysis of apoptosis induced by irradiation.

Thymuses from wild type B6 mice were harvested and frozen post-irradiation at the indicated times (1 hour through 24 hours). Sections were stained by TUNEL (green) to detect apoptotic cells and with anti-DEC205 (red) to mark the thymic epithelium. Panel labeled “control' was a section stained with secondary antibodies only. Panel labeled “w/o irr.” Was a section from a nonirradiated mouse. Original magnification was 200X.