Table 2. Constitutive and LPS induced IL-10 production by various murine B cell subsets.
| Anatomical location | B cell subset | Surface phenotype | IL-10 (pg/ml) | |
| Media | LPS | |||
| Peritoneum | B-1a cells | B220+Mac-1+CD5+ | 161±32 | 4063±406 |
| B-1b cells | B220+Mac-1+CD5− | 32±9 | 1193±126 | |
| B-2 cells | B220+Mac-1−CD5− | 3±1 | 337±166 | |
| Spleen | B-1 cells | B220+Mac-1−CD5+ | ND | 583±42 |
| Marginal Zone B cells | B220+AA4.1−CD21hi CD23lo | 9±5 | 1054±32 | |
| Follicular B cells | B220+AA4.1−CD21int CD23hi | 1±2 | 136±12 | |
| Total B cells | B220+ | ND | 313±26 | |
| Mesenteric Lymph Nodes | Total B cells | B220+ | 1±2 | 240±18 |
B cell subsets in the peritoneum, spleen and mesenteric lymph node were separated by FACS sorting (≥96% pure) and were cultured at 105 cells/well with or without 5 µg/ml LPS for 2 days. IL-10 was quantified by ELISA. ND = non-detectable.