Skip to main content
. 2010 Jul 6;5(7):e11060. doi: 10.1371/journal.pone.0011060

Figure 3. Figure 3 demonstrates chromatographic separation using Sephacryl SH500 that shows the separation of Francisella capsule from the LPS.

Figure 3

Panel A is a ProQ Emerald green stained SDS-PAGE showing the results of column separation. The capsule can be seen in lanes 13 and 14 and the LPS in lanes 21 and 23. Lane S is an aliquot of the sample after proteinase K and phenol extraction and prior to Triton X-114 extraction. Panel B is an immunodot assay of each fraction using MAb 11B7. The numbers in the panels correspond to aliquots from each one ml fraction from the column.