FIG. 6.

Increases in P-ERK expression in response to capsaicin (CAP) are repressed in rats fed cocoa enriched diets. Sections of the anterolateral portion (V1) of the ganglia were obtained from untreated animals (Control), animals injected with capsaicin (CAP) for two hours, or animals fed 10% cocoa (g/g) diet for 14 days and injected with capsaicin for 2 hours prior to harvesting the ganglia (Cocoa + CAP). A 400× magnification of neuron-satellite glia enriched regions stained for P-ERK is shown in the left panels. The next three panels represent the same section stained with the nuclear dye DAPI, a merged image showing both P-ERK and DAPI staining, and an enlarged image taken from the tissue outlined by the yellow box on the merged image (right panel). Neurons are identified by vertical arrows.