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Muc4 has no effect on ErbB3 phosphorylation. A375 Muc4-transfected (Rep 3 clone) cells with or without Muc4 (48 h) and starved (0.1% FBS) for 24 h were incubated at 37°C with HRG-1β [50 nM] for 3 min. Cleared lysates were loaded in triplicates and immunoblotted with anti-ErbB3, anti-ErbB Y1289, and anti β-actin antibodies.
