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Muc4 expression promotes cell migration signaling by increasing the phosphorylation of focal adhesion kinase (FAK). Immunoblot validation analyses of the microarry screen results. Proteins from RIPA extracts of A375 Muc4-transfected cells induced or not to express Muc4 (48 h) were immunoblotted with the indicated signaling antibodies. Normalized signal units were calculated using the ImageJ NIH densitometry software, and normalized with the corresponding β-actin singal.
