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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: J Cell Physiol. 2010 Sep;224(3):691–701. doi: 10.1002/jcp.22173

Figure 4. Src is an upstream signaling partner of Erk.

Figure 4

ROS osteoblast-like cells were cultured until they were 80% confluent and serum starved for 24 hours. Cells were pre-treated with PP2 (20mM), PD98059 (PD; 20mM) or DMSO (the diluent for PP2 or PD98059) for 30 minutes, and then treated with TGF-b1 (5ng/ml) for 20 minutes. After treatment, whole-cell protein extracts were analyzed by Western blot analysis. The inhibition of Src activation by PP2 also blocks the activation of Erk, whereas inhibition of Erk has no effect on Src activation by TGF-β1. Experiment was repeated 4 times with similar results.