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. 2010 Jun 4;41(5):63. doi: 10.1051/vetres/2010036

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© The authors, published by INRA/EDP Sciences, 2010

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any noncommercial medium, provided the original work is properly cited.

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Figure 1. — Cellular localisation of sortase A (SrtA) in Streptococcus uberis. Protein extracts were obtained from S. uberis 0140J and srtA mutant from cultures grown overnight in THB media. Fractions were separated on 15% SDS PAGE gels, transferred to nitrocellulose and probed with antiserum to SrtA (size of proteins of known molecular mass are indicated). Extracts were loaded as follows: from S. uberis 0140J capsule (lane 1); mutanolysin-derived cell wall (lane 3); cytoplasmic (lane 5); cell membrane (lane 7) and precipitated media extracts (lane 9) and from the srtA mutant capsule (lane 2); mutanolysin-derived cell wall (lane 4); cytoplasmic (lane 6); cell membrane (lane 8) and precipitated media extracts (lane 10). (A color version of this figure is available online at www.vetres.org.)