Figure 2.
The general gating strategy for identification of particular mononuclear phagocyte (MP) subpopulations (bone marrow is shown). Setups nos. 1–4 from Table II were used for identifying MP subpopulations. (A) The counts of all acquired leukocytes (with exclusion of cells debris) were determined (R0). Then the general gating strategy for mononuclear leukocytes (MNL) was applied for all setups. This included gating of mononuclear (R1) viable (R2) leukocytes in which the potentially contaminating neutrophils (SWC8+) were excluded (R3). (B) The CD172α+ (R5, R9) and CD172αhi (R4, R10) populations (a) were gated and CD172αhi/lo MP were calculated. The average from all setups, in which the CD172α staining was performed (setups nos. 1–3) was finally expressed. The counts of CD172αhi/lo CD14+/− (b, setup no. 1, R4, R5, R6) or CD172αhi/lo CD163+/− (c, setup no. 2, R10, R9, R7) or CD172αhi/lo MHCII+/− (d, setup no. 3, R10, R9, R8) MP were ascertained. The CD14+/− CD163+/− MNL (e, setup no. 4, R11, R12) were gated and their count was ascertained. Using the known counts of CD172αhi/lo CD14+/− and CD172αhi/lo CD163+/− (f), the counts of CD172α+ CD14+/− CD163+/− were finally calculated. (A color version of this figure is available online at www.vetres.org.)