Fig. 5.

Effect of auxin and cytokinin on IGI1 gene expression. Untreated 7 day old wild type seedlings were transferred to IAA and BAP treated medium and the seedlings were then used to test the hormone response within 3 h of treatment. There was no change in IGI1 gene expression in response to BAP treatment (d); however, IGI1 gene expression was down-regulated in response to exogenous IAA treatment, regardless of the concentration (a). The IAA5, CYP79B2, and ARR4 genes were used to verify the accuracy of the experimental method (b, c, and e). The actin transcript levels were used for normalization. Error bars indicate the standard deviation. The work was repeated two times with same result. Asterisks indicate values significantly different from the 0 uM (P < 0.05)