Table 1.
Plant/cross | Number of plants | ||
---|---|---|---|
Original mutant phenotype | Sterile plants | Dead plants | |
The original mutant/self | 226 | 119 | 150 |
The original mutant × wild type F1 F2 (7 plants) |
38 1638 |
0 910 |
31 941 |
Screened mutants did not have single T-DNA. After the mutant was back-crossed with Col-0, genotyping PCR was performed in the F2 generation to identify single T-DNA insertion lines. After fixing the original mutant, which contained a single T-DNA insertion, the mutant was back-crossed with Col-0 to determine if it was a single T-DNA insertion line or not. After seeding the heterozygous mutant (expected) in 1/2MS medium containing basta 20 mg/L, the plants were counted