Table 1.

Segregation ratio of the igi1 progeny

Plant/cross	Number of plants
	Original mutant phenotype	Sterile plants	Dead plants
The original mutant/self	226	119	150
The original mutant × wild type F1 F2 (7 plants)	38 1638	0 910	31 941

Screened mutants did not have single T-DNA. After the mutant was back-crossed with Col-0, genotyping PCR was performed in the F2 generation to identify single T-DNA insertion lines. After fixing the original mutant, which contained a single T-DNA insertion, the mutant was back-crossed with Col-0 to determine if it was a single T-DNA insertion line or not. After seeding the heterozygous mutant (expected) in 1/2MS medium containing basta 20 mg/L, the plants were counted