Figure 6.

Foxp3 is lost ‘preferentially’ by Il10rb^−/− T_reg cells in mice with colitis. (a) Ratio of CD45.1⁺ to CD45.2⁺ CD90.1⁻CD4⁺TCRβ⁺ cells isolated from spleen, PLNs, MLNs and LPL of Rag1^−/− recipients at 6 weeks after injection of 8 × 10⁵ C57BL/6 (CD90.1⁺) CD4⁺CD45RB^hi T cells, transferred with 2 × 10⁴ wild-type (CD45.1⁺) T_reg cells and 2 × 10⁴ Il10rb^−/− (CD45.2⁺) T_reg cells. Data are pooled from two independent experiments with a total of four mice (mean and s.d.). (b) Flow cytometry of Foxp3 expression by cells isolated from a Rag1^−/− recipient mouse as described in a, with gating on CD90.1⁻CD4⁺TCRβ⁺CD45.2⁻ cells (wild-type T_reg cells) or CD90.1⁻CD4⁺TCRβ⁺CD45.2⁺ cells (Il10rb^−/− T_reg cells). Bracketed lines indicate the Foxp3⁻ population. Data are representative of one of two independent experiments. (c) Foxp3⁻ cells in the CD90.1⁻CD4⁺TCRβ⁺CD45.2⁻ (wild-type T_reg) and CD90.1⁻CD4⁺TCRβ⁺CD45.2⁺ (Il10rb^−/− T_reg) populations isolated from Rag1^−/− mice as described in a. Each symbol represents an individual mouse; small horizontal bars indicate the mean. Data are pooled from two independent experiments with a total of four mice. (d) Foxp3⁻ cells in CD90.1⁻CD4⁺TCRβ⁺CD45.2⁻ (wild-type T_reg) or CD90.1⁻CD4⁺TCRβ⁺CD45.2⁺ (Il10rb^−/− T_reg) populations isolated from Rag1^−/− recipients of 4 × 10⁵ C57BL/6 (CD90.1⁺) CD4⁺CD45RB^hi T cells, transferred together with 1 × 10⁵ wild-type (CD45.1⁺) and 1 × 10⁵ Il10rb^−/− (CD45.2⁺) T_reg cells. Each symbol represents an individual mouse; small horizontal bars indicate the mean. * P < 0.01; ** P < 0.001 (two-tailed Student’s t-test). Data are pooled from two independent experiments with a total of four mice.