Figure 7.

Kinetics of IL-10 expression by T_reg cells and host cells. (a) GFP⁺ cells in IL-10 reporter mice. Numbers adjacent to outlined areas (top row) indicate percent GFP⁺ cells among gated naive splenocytes (TCRβ⁺CD4⁺CD45RB^hi) and T_reg splenocytes (TCRβ⁺CD4⁺CD45RB^loCD25⁺) from Il10^gfp mice; numbers in top right quadrants (bottom row) indicate percent CD45⁺GFP⁺ cells in tissues from Il10^gfpRag1^−/− mice. MFI, mean fluorescence intensity. (b) Flow cytometry of GFP⁺ cells in tissues 7 d after transfer of a mixture of CD45.1⁺ CD4⁺CD45RB^hi T cells and CD45.2⁺ Il10^gfp T_reg cells (ratio, 4:1). Top row, T_reg cells gated as CD45.2⁺ TCRβ⁺CD4⁺ cells; bottom row, gated TCRβ⁻CD4⁻ nonlymphoid cells. Numbers adjacent to outlined areas and in top right quadrants indicate percent GFP⁺ cells. (c) Real-time PCR analysis of Il10 mRNA in sorted T_reg cells (left), CD11b⁺ cells (middle) and CD11c⁺ dendritic cells (right) from various sites (keys) before transfer (0) or at 1, 2 and 6 weeks after transfer as in b. Data are from representative one of two independent experiments with six mice (a,b) or are pooled from two independent experiments with six mice (c; mean and s.d.).