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. Author manuscript; available in PMC: 2010 Sep 23.
Published in final edited form as: Sci Signal. 2010 Mar 23;3(114):ra22. doi: 10.1126/scisignal.2000818

Fig. 2. Characterization of endogenous InsP3R single channels in human B lymphoblasts.

Fig. 2

(A) Continuous single InsP3R channel current trace (300 s) recorded from outer membrane of nucleus isolated from human B lymphoblaste at +20 mV with 10 µM InsP3 and 1 µM free Ca2+ in pipette solution. Arrows indicate closed channel current levels. (B) Representative current traces (+20 mV) in nuclei isolated from human B lymphoblasts. Channel activity required presence of InsP3 (n = 20) and was inhibited by heparin (n = 15). (C) I/V relationship obtained by ramping holding potential from −60 to +60 mV. (D) Summary of InsP3R channel Po, and mean open τo and closed τc durations.