FIG. 8.

High-molecular-weight dextran permeates infected cell nuclei. (A and B) Mock- (A) and HCMV-infected (B) human fibroblasts were loaded with dextran-TRITC (green) at 48 hpi as described in Materials and Methods. Cells were examined for the presence of nuclear dextran at 72 (B, right two panels) and 96 hpi (B, left two panels). The position of the assembly compartment (AC) is indicated in the lower left panel of B. (C) Using the cell in the lower left panel of B, a z-axis series was generated, and slices through the cell from top to bottom are shown. The DAPI staining of the nucleus is included (blue).