FIG. 9.

Inhibition of dynein causes disruption of assembly compartment formation and loss of HCMV-mediated nuclear shape alterations. Human fibroblasts were infected for 24 h and then electroporated with a plasmid that expresses CC1-mCherry. The cells were plated on coverslips and incubated for an additional 48 h (72 hpi) and then prepared for immunofluorescence. CC1-expressing cells are indicated by the mCherry (red). Anti-pp28 was used to stain infected cells and indicate the status of the assembly compartments (green or yellow where it overlaps CC1). The shape and size of the nuclei were visualized with DAPI. The arrows show an uninfected cell expressing CC1 (CC1 Uninfected), an infected cell expressing CC1 (CC1 Infected), and an infected cell not expressing CC1 (Infected).