TABLE 2.
Methylation status of the promoter region of CDKN1A as determined by MSP
| Methylation status of CDKN1A promoter region | No. of samples by cell linea |
No. of primary samples by typeb |
|||
|---|---|---|---|---|---|
| T-ALL | HTLV-1-infected | ATLL | PBL | Acute-type ATLL | |
| Methylated | 3 | 0 | 2 | 0 | 6 |
| Partially methylated | 0 | 0 | 3 | 0 | 17 |
| Unmethylated | 0 | 3 | 1 | 5 | 1 |
| Total (% of methylation rates) | 3 (100) | 3 (0) | 6 (82) | 5 (0) | 24 (96) |
a
Cell lines used were three T-ALL cell lines (Jurkat, MOLT4, and MKB-1), three HTLV-1-infected T-cell lines (MT-2, MT-4, and HUT102), and six ATLL-derived cell lines (ED, SO4, Su9T01, S1T, KK1, and KOB).
b
For primary samples, PBLs were obtained from five healthy volunteers, and ATLL cells were from 24 patients with acute-type ATLL.