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. 2010 May 19;84(14):6923–6934. doi: 10.1128/JVI.00435-10

FIG. 5.

FIG. 5.

Identification of N-glycans that bind DC-SIGN. HIV vectors pseudotyped with 2.2, 2.2 ΔE2-196N, 2.2 ΔE1-139N, or 2.2 ΔE2-196N E1-139N were produced in the presence of DMNJ and designated 2.2 DMNJ, 2.2 ΔE2-196N DMNJ, 2.2 ΔE1-139N DMNJ, and 2.2 ΔE2-196N E1-139N DMNJ, respectively. The viruses were titrated on Jurkat cells by conjugating them with the antibody against HLA class I, which is abundantly expressed on Jurkat cells. The same titer of each virus was used to transduce DC-SIGN Jurkat cells without conjugation of an antibody. The p24 values of the viruses used for transduction are 2, 2, 8, and 8 ng for 2.2 DMNJ, 2.2 ΔE2-196N DMNJ, 2.2 ΔE1-139N DMNJ, and 2.2 ΔE2-196N E1-139N DMNJ pseudotype, respectively. EGFP expression was analyzed by flow cytometry 3 days posttransduction. The percentage of EGFP-positive cells is shown as an average of triplicate experiments with standard deviations. The y axis is forward scatter, and the x axis is EGFP expression.