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. 2010 Apr 28;84(14):7288–7299. doi: 10.1128/JVI.00535-10

FIG. 5.

FIG. 5.

Characterization of (pooled) escape viruses selected using PF-68742 and PF-4087738. NL4-3 viruses resistant to PF-68742 and PF-4087738 were generated by passaging breakthrough virus in the presence of increasing doses of compound. Two independent resistant populations were generated for each compound. (A) Dose-response curves are shown for each independent, resistant pool after amplification (n = 2) using either PF-68742 (top) or PF-4087738 (bottom). The passage control is NL4-3 virus passaged in parallel but in the absence of added compound and “start” virus is the original NL4-3 virus stock used for the experiment. (B) Cross-resistance study of the PF-68742 resistant (escape) virus and PF-4087738 resistant (escape) virus was performed. In addition, the T-20 sensitivity of breakthrough virus was assayed. NL4-3 viruses resistant to PF-68742 and PF-4087738 were treated with compound and the dose-response curves are shown for each independent, resistant pool after amplification (n = 2) using either PF-68742 (right) or PF-4087738 (center) or T20 (left) with corresponding passage controls and start NL4-3 virus.