FIG. 6.
Sensitivity of HIV-1 NL4-3G514R mutant to PF-68742, 5-helix, and T20. (A) Viral entry assays were performed with the original NL4-3 wild-type molecular clone and an engineered G514R point mutant in the presence of PF-68742, or the control inhibitors, 5-helix and T20. Experiments were performed in triplicate. The positions of the EC50s for the two PF-68742 dose-response curves are indicated by upward arrows. (B) P24 (left) and gp120 (right) ELISAs were performed on NL4-3 wild-type and NL4-3G514R mutant viruses used in panel B to verify that equivalent amounts of virus were used in the viral entry assays and that Env levels were unaffected by the mutation.