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. 2010 May 12;84(14):7225–7232. doi: 10.1128/JVI.00434-10

FIG. 4.

FIG. 4.

Quantitative real-time PCR and Western blot analysis on CD134 and CD9 expression. (A) Quantitative real-time PCR assessment on CD134 mRNA levels in various cell lines. The amount of CD134 mRNA in 104-C1-OrfA cells is approximately the same as that in 104-C1 cells. (B) Western blot against CD134 and CD9 in 104-C1 and 104-C1-OrfA cells. Lane 1, 104-C1 total cell lysate (7 μg); lane2, 104-C1-OrfA total cell lysate (7 μg); lane3, 104-C1 plasma membrane protein (2 μg); lane 4, 104-C1-OrfA plasma membrane protein (2 μg); lane 5, CrFK (upper panel) and 3201 (lower panel) plasma membrane protein (2 μg) as negative controls.