FIG. 6.

β-Gal assay for measurement of the entry of FIV-PPR or FIV-34TF10 envelope-pseudotyped virons. (A) pCFIV vectors pseudotyped with FIV-PPR Env were used to assess the degree of viral entry. A β-Gal assay performed at 48 h posttransduction shows that pCFIV pseudotyped with PPR Env infected GFox and 104-C1 cells more efficiently than it did CrFK and 104-C1-OrfA cells. RLU, relative luminescence units. (B) pCFIV vectors pseudotyped with 34TF10 Env were used to assess the viral entry for the same cell lines as those used for the above-described assay. The results showed that the viron infected CrFK cells more readily than it did GFox cells. On the other hand, no significant difference was observed for the 34TF10 Env-mediated viral entry between 104-C1 and 104-C1-OrfA cells.