FIG. 7.

A functional mLANA gene is required for the efficient replication of MHV68 following transfection of viral DNA in permissive fibroblasts. (A) NIH 3T12 fibroblasts were transfected with 0.5 μg of either 73.Stop-BAC or 73.MR-BAC (MR) DNA. At 3, 7, and 9 days posttransfection, supernatants were harvested, and titers of infectious virus produced were determined. The data are representative of at least three replicate experiments. (B) Vero cells were transfected in duplicate as described above, and lysates were harvested at 24, 72, and 96 h. Western blots for lytic MHV68 antigen and β-actin are shown.