FIGURE 3.

Loss of MV infection of EMT-induced II-18 cells. A and B, II-18 cells were infected with the retrovirus vector expressing Snail or control vector at an MOI of 50–100. Control and EMT-induced II-18 cells were infected with IC323-EGFP (A) or IC/Ed-H-EGFP (B). At 2 days p.i., the cells were observed under a phase-contrast or fluorescence microscope. C, control and EMT-induced II-18 cells were infected with IC323-Luci or IC/Ed-H-Luci. At 3 h p.i., the fusion block peptide was added to medium, and at 24 h p.i., the Renilla luciferase activity was measured. The activity in control cells was set to 100%. D, control and EMT-induced II-18 cells were incubated with medium containing control mouse IgG or a mAb against CD46 (M75) and infected with IC323-Luci. The procedures after MV infection were the same as those in C. E, CHO cells were transfected with pCA7ps-ICH or pCA7ps-EdH plus pCA7-ICF, using Lipofectamine 2000. Two days after transfection, the cells were harvested and cocultured with II-18 cells. After 2 days of incubation, the cells were subjected to Giemsa staining and observed under a light microscope. Error bars, S.D.