FIGURE 1.

Fiber formation of Rnq1p is faster at higher temperatures and enhanced by the addition of preformed seeds. Purified Rnq1PFD was diluted from denaturant (75-fold) to 4 μm, and fiber formation was followed using Th-T fluorescence. Graphs are plotted as % RFU versus time. A, Rnq1PFD unseeded at 25 °C (black squares) and 37 °C (gray circles). B, Rnq1PFD fiber formation at 25 °C unseeded (black squares), with 5% seeds formed at 25 °C (gray circles), and with 5% seeds formed at 37 °C (open triangles). C, Rnq1PFD fiber formation at 37 °C unseeded (black squares), with 5% seeds formed at 25 °C (gray circles) and with 5% seeds formed at 37 °C (open triangles). Graphs are representative of at least three independent trials.