FIGURE 5.

Variants of the [RNQ⁺] prion show distinct propagation and aggregation properties in vivo. A, mitotic stability of the [RNQ⁺] prion was dependent on the strength of the variant. The weak [RNQ⁺] variant lost the prion in ∼40% of the colonies (black bar), while medium [RNQ⁺] lost the prion in ∼19% of the colonies (white bar), and strong [RNQ⁺] lost the prion in <1% of the colonies (gray bar). Three independent experiments were performed for each variant to yield the resulting means and standard deviations. B, full-length endogenous Rnq1p in lysates from different [RNQ⁺] variants was fractionated by high speed centrifugation into total (T), supernatant (S), and pellet (P) fractions. The amount of protein in the supernatant fraction decreased as the strength of the variant increased. A representative blot from four independent experiments is shown.