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. 2010 Apr 30;285(27):20492–20498. doi: 10.1074/jbc.M110.102111

FIGURE 2.

FIGURE 2.

IL-10 inhibits miR-155 in primary BMDM and hPBMC. a, primary BMDM and hPBMC were stimulated with LPS (100 ng/ml) or LPS + IL-10 (20 ng/ml) and measured for miR-155 expression by RT-PCR. b, WT and IL-10-deficient BMDM (IL-10 KO) were untreated or pretreated with monoclonal (mAb) IL-10 antibody (5 μg/ml) for 1 h prior to stimulation with LPS (100 ng/ml) for 24 h. Cells were analyzed for IL-10 expression by enzyme-linked immunosorbent assay and for miR-155 expression by RT-PCR. In all cases, graphs are representative of at least three separate experiments. Error bars indicate S.D. Unstim, unstimulated.