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. 2010 Apr 30;285(27):20492–20498. doi: 10.1074/jbc.M110.102111

FIGURE 3.

FIGURE 3.

IL-10 inhibits the transcription of miR-155. a, IL-10-deficient BMDM were stimulated with LPS (100 ng/ml) or LPS + IL-10 (20 ng/ml). pri-miR-155, pre-miR-155, and mature miR-155 were measured by RT-PCR. b, Raw264.7 cells were co-transfected with wild-type BIC promoter luciferase plasmid and either si-control or si-STAT3. Cells were stimulated with LPS (100 ng/ml) or with LPS + IL-10 (25 ng/ml) for 18 h. Unstim, unstimulated. c, Raw264.7 cells transfected with wild-type BIC, NF-κB mutant (kB mut), AP1 mutant (AP1 mut), or Ets1 mutant (Ets1 mut) promoter luciferase plasmids were stimulated with LPS (100 ng/ml) or with LPS + IL-10 at two different doses (25 and 50 ng/ml). Luciferase activity was measured where results were normalized for TK-Renilla activity and represented as -fold stimulation over the non-stimulated control. In all cases, results are expressed as mean ± S.D. for triplicate determinations and are representative of three separate experiments.