FIGURE 6.

Effect of AbA on SLS activities in the cell-free system. TbSLSs were produced in the cell-free system using standard equimolar donor liposomes. A, proteoliposomes containing TbSLS1 (lanes 1 and 2), TbSLS2 (lanes 3 and 4), or TbSLS4 (lanes 5 and 6) were assayed in standard reactions for synthesis of NBD-SLs as in Fig. 4. Reactions were performed in the absence (−) or presence (+) of 500 nm AbA. B, products on TLC plates were quantified and are presented as percent of the AbA minus control (mean ± S.D., n = 3) for each TbSLS.