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. 2010 Apr 28;285(27):20664–20674. doi: 10.1074/jbc.M109.055608

FIGURE 5.

FIGURE 5.

TMEM59 localizes to the Golgi. A, endogenous TMEM59 was detected in permeabilized COS cells with polyclonal antibody 93 (green) and showed costaining with the endogenous Golgi marker giantin (red). As a control the TMEM59 antibody did not detect TMEM59 when blocked by the antigenic peptide. TMEM59 was detected in permeabilized COS cells with the polyclonal antibody 93 (red) and showed costaining with the endogenous Golgi marker p230 (green). B, endogenous TMEM59 was detected in permeabilized COS cells with the monoclonal antibody 4E5 (red) and showed a costaining with the endogenous Golgi marker giantin (green). An isotype antibody served as control for the specificity of the 4E5 antibody. C, GFP-tagged TMEM59 was transiently transfected into COS cells. Scale bars = 10 μm. D, HEK293 cells were transiently transfected with HA-TMEM59. Aliquots of the cell lysate were treated (+) or not (−) with endoglycosidase F or H as indicated. The actin blot shows equal loading in all lanes.