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. 2010 Apr 28;285(27):20664–20674. doi: 10.1074/jbc.M109.055608

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — A knockout of COG proteins shows similar defects of APP glycosylation as TMEM59 expression. A, wtCHO, ldlB-CHO, and ldlC-CHO cells were cotransfected with APP695 and either control plasmid (con) or TMEM59. The lysates (lys) were blotted against cellular APP (22C11) and TMEM59 (93), the supernatant (sup) was blotted against secreted APPsα (W02). The quantification shows the secreted APPsα normalized to the cellular APP and is represented as mean ± S.E. of three to four independent experiments. B, wtCHO, ldlB-CHO, and [ldlB]-CHO cells were transfected with APP695. [ldlB]-CHO cells are COG1-deficient ldlB cells retransfected with COG1. The lysates (lys) were blotted against cellular APP (22C11) and the supernatants (sup) against secreted APPsα (W02).